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Friday, July 24, 2020 | History

2 edition of use of reovirus type 3 protein sigma one in the therapy of murine EL4 lymphoma found in the catalog.

use of reovirus type 3 protein sigma one in the therapy of murine EL4 lymphoma

Mary Bosch Farone

use of reovirus type 3 protein sigma one in the therapy of murine EL4 lymphoma

by Mary Bosch Farone

  • 11 Want to read
  • 28 Currently reading

Published .
Written in English

    Subjects:
  • Reoviruses,
  • Lymphomas,
  • Microbiology

  • Edition Notes

    Statementby Mary Bosch Farone
    The Physical Object
    Paginationvi, 140 leaves :
    Number of Pages140
    ID Numbers
    Open LibraryOL14970783M

    Histiocytic sarcomas represent malignant tumors which require new treatment strategies. Canine distemper virus (CDV) is a promising candidate due to its oncolytic features reported in a canine histiocytic sarcoma cell line (DH82 cells). Interestingly, the underlying mechanism might include a dysregulation of angiogenesis. Based on these findings, the aim of the present study was to . Section 1 Malignant hematology and hematopoietic cell transplantation -- Part 1 Acute lymphoblastic leukemia in adults -- Part 2 Acute myeloid leukemia in adults -- Part 3 Myelodysplastic syndromes and related disorders -- Part 4 Myeloproliferative neoplasms -- Part 5 Chronic lymphocytic and other leukemias -- Part 6 Hodgkin Lymphoma -- Part 7.

    The overexpression of procaspase-3 represents a common alteration in cancer cells that can be exploited therapeutically. Procaspase-3 overexpression has been observed in lymphomas, melanoma, lung and breast cancers, among many others. Procaspase-3 is the zymogen form of caspase-3, a key executioner of apoptosis and responsible for the cleavage of over a hundred cellular by: Protein sets from fully sequenced genomes. Annotation systems. Systems used to automatically annotate proteins with high accuracy: UniRule (Expertly curated rules) Taxonomy - Reovirus type 2 (strain D5/Jones) (T2J) (Mammalian orthoreovirus 2)))) Map to.

    REOVIRUS MAMMALIAN ORTHOREOVIRUS 1 (ATCC VR) REOVIRUS TYPE 2 (ATCC VR) REOVIRUS 3 (ATCC VR) PATHOGEN SAFETY DATA SHEET / INFECTIOUS SUBSTANCES INFECTIOUS AGENT NAME: Reovirus GENERAL: Reoviridae is a family of viruses that can affect the gastrointestinal system and respiratory tract. Viruses in the family Reoviridae. Despite the improvement of cancer therapy in the past years, in many cases, cancer has remained an incurable disease [].In the last decades, it has become clear that the immunological tumor environment is crucial for the efficacy of many, if not all cancer therapies [2,3,4,5].This is demonstrated by the strong prognostic value of tumor infiltrating lymphocytes (TILs) for the patient’s Author: Mühlberger M, Unterweger H, Lehmann C, Heger L, Dudziak D, Alexiou C, Lee G, Janko C.


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Use of reovirus type 3 protein sigma one in the therapy of murine EL4 lymphoma by Mary Bosch Farone Download PDF EPUB FB2

J Virol. May;61(5) Inhibition of reovirus type 3 binding to host cells by sialylated glycoproteins is mediated through the viral attachment by: Abstract. In this report, we study the reovirus serotype 3 (strain Dearing) sigma 1 protein obtained from various sources: from Escherichia coli expressing sigma 1 protein, from reovirus-infected mouse L cells, and from purified by: Reoviruses are important models for studies of viral pathogenesis; however, the mechanisms by which these viruses produce cytopathic effects in infected cells have not been defined.

In this report, we show that murine L (L) cells infected with prototype reovirus strains type 1 Lang (TIL) and type 3 Dearing (T3D) undergo apoptosis and that T3D induces apoptosis to a substantially greater extent Cited by: The reovirus type 3 receptor is expressed predominantly on murine Lyt-2,3+ and human T8+ cells.

Epstein RL, Finberg R, Powers ML, Weiner HL. Reovirus type 3 binds to approximately 20% of murine and human T cells via the viral hemagglutinin, a small outer capsid by: 8. All strains produced the illness in micecharacteristic of reovirus type 3 and are neutralized by antiserum against one strain, CAN A certain degree of antigenic heterogeneity is 3, of from from cases of Burkitt's lymphoma in F.

a (Burkitt, ). of cases; case 18 June Cited by: VIROLOGY() Reovirus Type 3 Synthesizes Proteins in Interferon-Treated HeLa Cells without Reversing the Antiviral State ELENA FEDUCHI,* MARIANO ESTEBAN.+ AND LUIS CARRASCO*'1 *Centro de Biologie Molecular, Universidad Automa de Madrid, Canto Blanco, Madrid, Spain, and ^Department of Biochemistry, Downstate Medical Center, Clarkson Avenue, Cited by: 7.

The sigma 3 protein of reovirus serotype 3 is present in different forms differing in isoelectric point, as already described by other authors. Proteolytic digestion of each of these forms generates similar peptides. The different forms are present in the mature virus and have affinity for double-stranded RNA and for by: 1.

The reovirus σ1 protein is encoded by the S1 gene segment and is probably the most extensively studied of all reovirus proteins. This is mainly because σl is the reovirus cell attachment protein and, as such, represents the first viral protein the cell encounters during reovirus by: J.D.

Chappell, V.L. Gunn, J.D. Wetzel, G.S. Baer, T.S. DermodyMutations in type 3 reovirus that determine binding to sialic acid are contained in the fibrous tail domain of viral attachment protein ς1Cited by: Reovirus infection induces the formation of large cytoplasmic inclusions that serve as the major site of viral assembly.

Reovirus strains type 3 Dearing (T3D) and type 1 Lang (T1L) differ in the rate of inclusion formation in L cells. The median time of inclusion formation is 18 h in cells infected with T3D and 39 h in cells infected with by: Featured community.

Creative Data Solutions (CDS) is a Vanderbilt Shared Resource and has extensive experience in providing effective and robust solutions to challenges pertaining to research data using modern informatics and bioinformatics approaches.

Reovirus type-1 selectively adheres to M-cells by interacting with α 3-linked sialic acid glycoconjugates expressed by M-cell [86]. The infection causes a depletion of the M-cells from the FAE. Most of the more recent clinical studies are carried out with the reovirus Type 3 Dearing (T3D) strain [5,6].

A third reovirus serotype (Type 1 Lang; T1L) is frequently used in comparative studies with reovirus T3D, especially those concerning the mechanisms of infection and replication in cell lines, and the pathogenesis in mouse models [7,8,9].Cited by: 9.

One of the best characterized models of reovirus pathogenesis is infection of the murine central nervous system (CNS), in which serotype 1 (T1) and serotype 3 (T3) reoviruses display markedly. (vi) Reovirus type 3.

Mammalian reoviruses are grouped into serotypes 1, 2, and 3. Reovirus type 3 is the most pathogenic reovirus of laboratory rodents. The primary importance of reovirus type 3 is as a contaminant of transplantable tumors and cell lines (, ).

Reovirus type 3 is a dsRNA virus in the family Reoviridae. Transmission is Cited by: Immunotherapy of EL4 lymphoma with reovirus. Article. Nov stability in which the dsRNA genome is protected not only by tight sigma 3-mu 1, lambda 2-sigma 3, and lambda 2-mu 1.

Role of the S1 Gene Segment in Reovirus-Induced Apoptosis. The S1 gene segment encodes the viral attachment protein σ1 and the nonstructural protein σ1s.σ1s is the determinant of reovirus-induced G 2 /M cell cycle arrest, an effect that results from inhibition of the G 2 /M regulatory kinase p34 cdc2 and the resulting inhibition of cellular DNA synthesis (Tyler et al.

; Poggioli et. Cells (1 10 5) were infected with reovirus strain T3D at an MOI of 1 PFU per cell. After adsorption for 1 h, the inoculum was removed, fresh medium was added, and the cells were incubated at Structural model of protein p The first open reading frame of the avian reovirus S1 gene expresses a residue nonstructural protein designated p p10 is a type-1 transmembrane protein, which contains a fusion sequence on its N-terminal ectodomain and a Cited by: We evaluated the timing and infection cycle of reovirus type-3 strain Dearing (T3D), using one-step replication experiment by molecular and conventional methods in MSCs and L cell as control.

Reoviruses are non-enveloped and have an icosahedral capsid composed of an outer (T=13) and inner (T=2) protein shell. The genomes of viruses in Reoviridae contain 10–12 segments which are grouped into three categories corresponding to their size: L (large), M (medium) and S (small).

Segments range from about to 3 kbp and each segment encodes 1–3 proteins (10–14 proteins in total).(unranked): Virus. Identification of novel O-linked glycosylated residues in Ncr1.

Ncr1 is predicted to have three N-linked glycosylations at positions N, N and Cited by: 6.EL4 cells were labelled with mouse CD4 and CD8 antibody conjugates, followed by labelling murine PD-1 antibody conjugate.

The FACS data shows that % CD4+ EL4 cells and % CD8+ EL4 cells were PD-1 positive cells (Figure 3A). Overall, EL4 cells had a relatively high percentage of cells that were PD-1 Cited by: 5.